TRANSDUCTION OF BACILLUS LICHENIFORMIS AND BACILLUS SUBTILIS BY EACH OF TWO PHAGES
- 1 September 1963
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 86 (3), 452-+
- https://doi.org/10.1128/jb.86.3.452-461.1963
Abstract
A second transducing bacteriophage, designated SP-15, was isolated from the same soil-sample culture filtrate that supplied the Bacillus subtilis transducing phage, SP-10, reported earlier from this laboratory. SP-10 and SP-15 differ serologically and in several other respects, but share the ability to propagate on B. subtilis W-23-Sr (streptomycin-resistant) and B. licheniformis ATCC 9945a, and to mediate general transduction in either species when propagated homologously. Attempts to transduce between the species have failed. SP-10 forms plaques readily on both W-23-Sr and 9945a; SP-15 forms minute plaques on W-23-Sr and has shown no evidence of any lytic activity on 9945a. Maximal recoveries of prototropic colonies from mixtures of SP-10 with suxotrophs of either W-?.3-Sr or 9945a were obtained only when excess phage was neutralized by post-transduction treatment with specific phage antiserum. Such treatment was not necessary for maximal recovery of transductants effected by SP-15. Unlike SP-10, SP-15 propagated on W-23-Sr did not transduce B. subtilis 168 (indole"). SP-15 transduced B.licheniformis more efficiently than did SP-10. Neither phage was able to transduce B. licheniformis as efficiently as it transduced B. subtilis. The differing influences of multiplicity of infection were compared for the two phages in both species.Keywords
This publication has 10 references indexed in Scilit:
- Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperatureJournal of Molecular Biology, 1962
- Transformation of genetic traits by the DNA prepared from a lysogenic phage S-1Biochemical and Biophysical Research Communications, 1962
- Genetic transformation of Bacillussubtilis by extracellular DNABiochemical and Biophysical Research Communications, 1962
- Some characteristics of subtilis phages with transducing activityThe Science of Nature, 1962
- TRANSDUCTION IN BACILLUS SUBTILISJournal of Bacteriology, 1962
- Chelation therapy in circulatory and sclerosing diseases.1961
- Genetic transduction in BacillussubtilisBiochemical and Biophysical Research Communications, 1961
- Isolation and Classification of a New Series of Azotobacter BacteriophagesJournal of General Microbiology, 1961
- PRODUCTION OF GLUTAMYL POLYPEPTIDE BY BACILLUS SUBTILISJournal of Bacteriology, 1954