Mumps Meningo-Encephalitis. Isolation in Chick Embryos of Virus from Spinal Fluid of a Patient.

Abstract

Two strains of mumps virus were isolated from the spinal fluids of patients with meningo-encephalitis without parotitis by amniotic inoculation of 8-day-old chick embryos. In one of the cases, described in detail, positive hemagglutination was obtained with amniotic fluid of the 1st passage on the 5th day of incubation. The virus was specifically neutralized by immune and convalescent sera as measured by inoculation of chick embryos; it could be used as antigen for complement-fixation tests with acute and convalescent mumps sera; and it produced parotitis in exposed individuals in contrast to the strain available in the laboratory at that time, which gave only subclinical infections.