The nucleotide sequence of cea* and the region of origin of plasmid pKY-1.
- 1 January 1986
- journal article
- research article
- Published by Microbiology Research Foundation in The Journal of General and Applied Microbiology
- Vol. 32 (5), 433-442
- https://doi.org/10.2323/jgam.32.433
Abstract
We have constructed a restriction map of the colicinogenic plasmi pKY-1 and analyzed its genetic structure. Although the cleavage map of pKY-1 is completely different from that of ColE1, the arrangement of its genes cea* and imm* and the region of origin (replication origin) is the same as that of ColE1. The nucleotide sequence of the region of origin and of the cea*, imm* and kil* genes were determined. The sequence of the region of origin is almost identical with that of ColE1. The sequence of the RNAI deduced from the sequence is a hybrid of ColE1 and CloDF13 consistent with the fact that pKY-1 is compatible with ColE1. The amino acid sequence of colicin E1* deduced from the nucleotide sequence is almost identical with that of colicin E1. However, significant substitutions of amino acids in colicin E1* were observed in the region which would correspond to the N-M junction of oclicin E1.Keywords
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