Quantitative Microscopic Approaches for Studying Kidney Functions
- 1 March 2006
- journal article
- review article
- Published by S. Karger AG in Nephron Physiology
- Vol. 103 (2), p63-p70
- https://doi.org/10.1159/000090621
Abstract
Intravital ratiometric microscopy is a powerful method for quantitative study of kidney functions. As demonstrated in the examples, both the generalized polarity and direct ratio imaging approaches allow investigators to address basic and important questions such as those related to filtration, permeability and reabsorption. Both approaches have similar advantageous for in vivo imaging. However, they have different sensitivities and responses to the component intensity changes. Therefore, investigators must design their experiments based on the characteristics of the generalized polarity function and direct ratio approach.Keywords
This publication has 15 references indexed in Scilit:
- Quantitative intravital microscopy using a Generalized Polarity concept for kidney studiesAmerican Journal of Physiology-Cell Physiology, 2005
- Intravital multiphoton microscopy of dynamic renal processesAmerican Journal of Physiology-Renal Physiology, 2005
- Minocycline reduces renal microvascular leakage in a rat model of ischemic renal injuryAmerican Journal of Physiology-Renal Physiology, 2005
- Uptake and trafficking of fluorescent conjugates of folic acid in intact kidney determined using intravital two-photon microscopyAmerican Journal of Physiology-Cell Physiology, 2004
- Injury of the renal microvascular endothelium alters barrier function after ischemiaAmerican Journal of Physiology-Renal Physiology, 2003
- Intravital Imaging of the Kidney Using Multiparameter Multiphoton MicroscopyNephron Experimental Nephrology, 2003
- Microvascular endothelial injury and dysfunction during ischemic acute renal failureKidney International, 2002
- The Rat Glomerular Filtration Barrier Does Not Show Negative Charge SelectivityMicrocirculation, 2002
- Functional studies of the kidney of living animals using multicolor two-photon microscopyAmerican Journal of Physiology-Cell Physiology, 2002
- Fluorescence generalized polarization of cell membranes: a two-photon scanning microscopy approachBiophysical Journal, 1996