Polyamine metabolism in a rat brain tumor cell line: Its relationship to the growth rate
- 1 December 1975
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 86 (3), 511-521
- https://doi.org/10.1002/jcp.1040860308
Abstract
To investigate whether the metabolism of the polyamines putrescine, spermidine and spermine is related to cellular growth rate, we have measured the activities of L-ornithine decarboxylase and S-adenosyl-L-methionine decarboxylase as well as the levels of the polyamines in rat brain tumor cells at various stages of a 7-day in vitro growth period and correlated them with the continuous changes in specific growth rate ([dN[t]/dt]/N[t]). L-Ornithine decarboxylase and S-adenosyl-L-methionine decarboxylase both exhibited their maximal activities at the time of most rapid growth. A high positive correlation between the activities of these enzymes and the specific growth rate of the tumor cells during the entire growth period was demonstrated statistically. The pattern of fluctuation of the spermidine content during the culture cycle was similar to those of the enzyme activities and likewise showed a high positive correlation with the specific growth rate of the tumor cells during the entire growth period. The putrescine content exhibited a low positive correlation, whereas the spermine content exhibited a somewhat higher, but negative correlation with the specific growth rate. The high correlation between the specific growth rate of the tumor cells and the synthesis of the polyamines indicates that these events are primarily associated with processes involved in cell replication. Putrescine and spermidine are thought to participate in the regulation of cellular growth rate; a high content may augment, and a low content may restrain, cellular growth rate.Keywords
This publication has 16 references indexed in Scilit:
- An automated micromethod for the quantitative analysis of di- and polyamines utilizing a sensitive high pressure liquid chromatographic procedureFEBS Letters, 1974
- Reciprocal changes in the levels of functionally related folate enzymes during the culture cycle in human fibroblastsBiochemical and Biophysical Research Communications, 1973
- New Aspects of Polyamine Biosynthesis in Eukaryotic OrganismsAdvances in Enzyme Regulation, 1972
- Diurnal change in ornithine decarboxylase activity of rat liverBiochemical and Biophysical Research Communications, 1972
- Separation of enzyme activities catalysing spermidine and spermine synthesis in rat brainFEBS Letters, 1971
- Morphological and immunochemical studies of rat glial tumors and clonal strains propagated in cultureJournal of Neurosurgery, 1971
- Separation of two proteins required for synthesis of spermidine from S-adenosyl-L-methionine and putrescine in rat prostateBiochemical and Biophysical Research Communications, 1971
- Mechanisms and regulation of polyamine and putrescine biosynthesis in male genital glands and other tissues of mammalsAdvances in Enzyme Regulation, 1969
- Stimulation of the decarboxylation of S-adenosylmethionine by putrescine in mammalian tissuesBiochemical and Biophysical Research Communications, 1968
- Tumors of the Central Nervous System in Monolayer TissueArchives of Neurology, 1966