Rapid and Highly Sensitive Detection of Lead Ions in Drinking Water Based on a Strip Immunosensor
Open Access
- 27 March 2013
- Vol. 13 (4), 4214-4224
- https://doi.org/10.3390/s130404214
Abstract
In this study, we have first developed a rapid and sensitive strip immunosensor based on two heterogeneously-sized gold nanoparticles (Au NPs) probes for the detection of trace lead ions in drinking water. The sensitivity was 4-fold higher than that of the conventional LFA under the optimized conditions. The visual limit of detection (LOD) of the amplified method for qualitative detection lead ions was 2 ng/mL and the LOD for semi-quantitative detection could go down to 0.19 ng/mL using a scanning reader. The method suffered from no interference from other metal ions and could be used to detect trace lead ions in drinking water without sample enrichment. The recovery of the test samples ranged from 96% to 103%. As the detection method could be accomplished within 15 min, this method could be used as a potential tool for preliminary monitoring of lead contamination in drinking water.Keywords
This publication has 28 references indexed in Scilit:
- Colloidal gold nanoparticle probe-based immunochromatographic assay for the rapid detection of chromium ions in water and serum samplesAnalytica Chimica Acta, 2012
- Risk assessment of the lead intake by consumption of red deer and wild boar meat in Southern SpainFood Additives & Contaminants: Part A, 2011
- Pb2+-introduced activation of horseradish peroxidase (HRP)-mimicking DNAzymeChemical Communications, 2011
- Recent Trends in Macro-, Micro-, and Nanomaterial-Based Tools and Strategies for Heavy-Metal DetectionChemical Reviews, 2011
- Ultrasensitive detection of lead(ii) with DNAzyme and gold nanoparticles probes by using a dynamic light scattering techniqueChemical Communications, 2011
- Immunochromatography for the rapid determination of cadmium concentrations in wheat grain and eggplantJournal of the Science of Food and Agriculture, 2011
- Separation, preconcentration and inductively coupled plasma-mass spectrometric (ICP-MS) determination of thorium(IV), titanium(IV), iron(III), lead(II) and chromium(III) on 2-nitroso-1-naphthol impregnated MCI GEL CHP20P resinJournal of Hazardous Materials, 2010
- Determination of trace elements in food samples by ICP-AES after preconcentration with p-toluenesulfonylamide immobilized on silica gel and nanometer SiO2Microchimica Acta, 2007
- Characterization of Monoclonal Antibodies for Lead−Chelate Complexes: Applications in Antibody-Based AssaysJournal of Agricultural and Food Chemistry, 2007
- Identification of Important Residues in Metal−Chelate Recognition by Monoclonal AntibodiesBiochemistry, 2003