Effects of soluble metals on human peri‐implant cells
- 3 June 2005
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part A
- Vol. 74A (1), 124-140
- https://doi.org/10.1002/jbm.a.30345
Abstract
Despite reports associating tissue necrosis with implant failure, the degree to which processes, such as metal toxicity, negatively impact implant performance is unknown. We evaluated representative human peri-implant cells (i.e., osteoblasts, fibroblasts, and lymphocytes) when challenged by Al+3, Co+2, Cr+3, Fe+3, Mo+5, Ni+2, and V+3 chloride solutions (and Na+2 as a control) over a wide range of concentrations (0.01–10.0 mM). Cell responses were measured using proliferation assays, viability assays, and microscopic cell morphology assessments. Differential effects were found to be less a function of the cell type than of the composition and concentration of metal challenge. No preferential immunosuppression was demonstrated. Below 0.01 mM, no metal was toxic. The most toxic metals (i.e., Co, Ni, and V) reduced proliferation (IC50), and viability (LC50) and cell morphology of osteoblasts, fibroblasts, and lymphocytes by M to exact the same responses. Below 1 mM, these toxic metals also induced alterations in all cell morphology consisting of loss of filopodia or lamellipodia or changes in cell shape. Metals that were toxic at clinically relevant concentrations (less than previously reported values in peri-implant tissues/fluids) include Co (0.6 mM), Ni (0.8 mM), V (0.5 mM) for lymphocytes and Co (0.8 mM), V (0.3 mM), Al (1–5 mM), Fe (1–5 mM) for fibroblasts, and Co (0.8 mM), Ni (0.7 mM), V (0.1 mM) for osteoblasts. Only Co and V were toxic in vitro at concentrations below that detected in vivo in synovial fluid (V at 0.1 mM and Co at 0.8 mM for fibroblasts, and V at 0.4 mM and Co at 0.8 mM on osteoblasts). Thus, soluble Co and V released from Co- and Ti-based alloys, respectively, could be implicated as the most likely to mediate cell toxicity in the periprosthetic milieu. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005Keywords
This publication has 43 references indexed in Scilit:
- Interfacial kinetics of titanium‐ and cobalt‐based implant alloys in human serum: Metal release and biofilm formationJournal of Biomedical Materials Research Part A, 2003
- Metal-On-Metal Hip Replacements: Wear Performance and Cellular Response To Wear ParticlesPublished by ASTM International ,1999
- Effect of metal ions on the formation and function of osteoclastic cellsin vitroJournal of Biomedical Materials Research, 1997
- Crevice Corrosion of Cemented Titanium Alloy Stems in Total Hip ReplacementsClinical Orthopaedics and Related Research, 1996
- Tissue Reaction to Metal on Metal Total Hip ProsthesesClinical Orthopaedics and Related Research, 1996
- Effects of sublethal metal ion concentrations on osteogenic cells derived from bone marrow stromal cellsJournal of Applied Biomaterials, 1995
- Acute toxicity of metal ions in cultures of osteogenic cells derived from bone marrow stromal cellsJournal of Applied Biomaterials, 1995
- Metallic wear debris in metal‐backed patellar failureJournal of Applied Biomaterials, 1994
- The in vitro toxicity of cobalt-chrome-molybdenum alloy and its constituent metalsBiomaterials, 1986
- Serum concentrations of chromium, cobalt and nickel after total hip replacement: a six month studyBiomaterials, 1983