The physical state of HPV16 infection and its clinical significance in cancer precursor lesion and cervical carcinoma
Open Access
- 14 May 2008
- journal article
- research article
- Published by Springer Science and Business Media LLC in Zeitschrift für Krebsforschung und Klinische Onkologie
- Vol. 134 (12), 1355-1361
- https://doi.org/10.1007/s00432-008-0413-3
Abstract
Purpose Integration of high-risk human papillomavirus (HR-HPVs) into the host DNA has been proposed as a risk for cervical carcinogenesis. HPV-16 is the predominant high-risk type and its integration ration varied largely in different cervical cancer (CC) samples. The aim of this study was to evaluate the correlation between physical state of HPV16 infection and extent of cervical lesion, as well as the clinical significance of virus existing state. Methods A total of 252 cases of paraffin-embedded blocks derived from cancer precursor lesion and cervical carcinoma samples were detected by HC-II for HR-HPV infection. HPV16 infection was confirmed by PCR and immunohistochemistry for HPV16 E7 simultaneously. The physical state of HPV16 infection were assessed by PCR for 3 overlapping fragments in E2 gene and multiple PCR for E2 gene and E7 gene. Results The infection ratio of HR-HPV in normal cervical tissue, cervical intraepithelial neoplasia (CIN) I, CIN II, CIN III and cervical cancer were 15.0, 32.8, 54.3, 69.7, 93.8%, respectively. HR-HPV positive samples of 62.8% were infected with HPV16. The integration ratio of HPV16 in CIN III and cervical carcinoma were 35.7 and 58.1% respectively, both of which were significantly higher than that of CIN I and normal cervical tissues. The discrepancy was statistically significant ( P < 0.05). Furthermore, it was observed that persistent virus infection and progression of cervical lesion were more common in CIN I with integrated HPV16 than that with episomal HPV16. Conclusion The integration ratio of HPV16 was accompanied by an increase in the grade of cervical lesion. The integrated state of HPV16 infection was strongly associated with persistent HPV infection and progression of cervical lesions.Keywords
This publication has 28 references indexed in Scilit:
- Early integration of high copy HPV16 detectable in women with normal and low grade cervical cytology and histologyJournal of Clinical Pathology, 2006
- Interlaboratory Reliability of Hybrid Capture 2American Journal of Clinical Pathology, 2004
- Polymorphisms in Interleukin-1β and Interleukin-1 Receptor Antagonist Genes Are Associated with Kidney Failure in Korean Patients with Type 2 Diabetes mellitusAmerican Journal of Nephrology, 2004
- Interlaboratory Reliability of Hybrid Capture 2American Journal of Clinical Pathology, 2004
- Epidemiologic Classification of Human Papillomavirus Types Associated with Cervical CancerNew England Journal of Medicine, 2003
- Integrated Human Papillomavirus Type 16 Is Frequently Found in Cervical Cancer Precursors as Demonstrated by a Novel Quantitative Real-Time PCR TechniqueJournal of Clinical Microbiology, 2002
- Prevalence of Human Papillomavirus in Cervical Cancer: a Worldwide PerspectiveJNCI Journal of the National Cancer Institute, 1995
- Human papillomaviruses in the pathogenesis of anogenital cancerVirology, 1991
- Association of Human Papillomavirus Types 16 and 18 E6 Proteins with p53Science, 1990
- Behavior of Mild Cervical Dysplasia During Long-Term Follow-upObstetrics & Gynecology, 1986