Species distribution, genetic diversity and antimicrobial susceptibility of Campylobacter isolates recovered from the preputial cavity of healthy rams in Turkey
Open Access
- 16 May 2020
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 129 (5), 1173-1184
- https://doi.org/10.1111/jam.14703
Abstract
Aims Campylobacter spp. are important causes of reproductive disease in ruminants worldwide. Although healthy bulls are well‐known carriers for infection of cows, the role of rams as a potential source for infecting ewes is unclear. This study aimed to determine prevalence, species distribution, genetic diversity and antimicrobial susceptibility profiles of Campylobacter spp. isolated from the preputial cavity of healthy rams. Methods and Results The material of this prospective study comprised 191 swab samples taken from the preputial cavity of healthy rams. Enrichment and membrane filtration were employed for the isolation of Campylobacter. Presumptive isolates were confirmed as Campylobacter by phenotypic and molecular tests. 16S rRNA gene sequence analysis was used for the definitive identification of the isolates at species level, and genotyping was performed using pulsed‐field gel electrophoresis (PFGE). The susceptibility of the Campylobacter spp. isolates to various antibiotics was determined by the disk diffusion test. Twenty‐seven of the 191 (14.13%) swab samples were found to be positive for Campylobacter spp. (28 isolates were recovered in total). Per phenotypic and genotypic analyses, one isolate was identified as Campylobacter mucosalis and the remaining 27 isolates were identified as Campylobacter sputorum bv. faecalis. The PFGE analysis of the C. sputorum biovar faecalis isolates produced 17 clusters and 24 different pulsotypes, indicating high genetic heterogeneity. All 28 isolates were found to be susceptible to all of the antibiotics tested. Conclusions Healthy rams may be an important reservoir of different Campylobacter species in the preputium. Significance and Impact of the Study This study demonstrated for the first time that healthy rams can carry different Campylobacter spp. including genetically diverse C. sputorum bv. faecalis and C. mucosalis in the preputial cavity. Further investigation on the potential implication of this finding on sheep reproductive health (e.g., infectious infertility, and abortion) and overall epidemiology of Campylobacter may be warranted.Keywords
This publication has 55 references indexed in Scilit:
- The clinical importance of emerging Campylobacter speciesNature Reviews Gastroenterology & Hepatology, 2011
- Campylobacter troglodytis sp. nov., Isolated from Feces of Human-Habituated Wild Chimpanzees ( Pan troglodytes schweinfurthii ) in TanzaniaApplied and Environmental Microbiology, 2011
- The Human Oral MicrobiomeJournal of Bacteriology, 2010
- Detection and quantification of 14 Campylobacter species in pet dogs reveals an increase in species richness in feces of diarrheic animalsBMC Microbiology, 2010
- Development of cpn60 -Based Real-Time Quantitative PCR Assays for the Detection of 14 Campylobacter Species and Application to Screening of Canine Fecal SamplesApplied and Environmental Microbiology, 2009
- Postglacial connection of the Black Sea to the Mediterranean and its relation to the timing of sapropel formationPaleoceanography and Paleoclimatology, 1997
- Campylobacter upsaliensis, C. sputorum sputorum and C. concisus as Common Causes of Diarrhoea in Swedish ChildrenScandinavian Journal of Infectious Diseases, 1995
- Attempted experimental infection of the preputial cavity of three male goats with Campylobacter speciesPublished by Wiley ,1988
- Ovine abortion associated with Campylobacter jejuniPublished by Wiley ,1986
- Some Features of Campylobacter sputorum subsp. mucosalis subsp. nov., nom. rev. and Their Taxonomic SignificanceInternational Journal of Systematic and Evolutionary Microbiology, 1981