Cross‐resistance, fitness costs, and biochemical mechanism of laboratory‐selected resistance to tenvermectin A in Plutella xylostella
- 4 February 2021
- journal article
- research article
- Published by Wiley in Pest Management Science
- Vol. 77 (6), 2826-2835
- https://doi.org/10.1002/ps.6317
Abstract
BACKGROUND Tenvermectin A is a new avermectin derivative that has good insecticidal and acaricidal effects. In order to study the resistance of Plutella xylostella to tenvermectin A, a sensitive strain (SS) and a laboratory‐selected tenvermectin A‐resistant strain (RS, 33.57‐fold) were used to evaluate cross‐resistance and fitness costs as well as to determine the resistance mechanism. RESULTS There was no cross‐resistance with common pesticides except for moderate cross‐resistance with cypermethrin (resistance ratio = 10.26‐fold) observed in RS. The activities of metabolic enzymes were measured, and the results showed that mixed function oxidase (MFO) and carboxylate esterase (CarE) in RS increased significantly by 2.92‐ and 2.86‐fold, respectively, compared with SS. In addition, there was no obvious difference in glutathione‐S‐transferase (GST), which indicated that enhanced MFO and CarE activities may be the main mechanisms of detoxification. In the four typical resistance‐related genes, expression of GluCl (4.86‐fold), ABCC2 (3.85‐fold), and CYP6 (2.94‐fold) in RS were significantly promoted, but expression of GST was not. The clone and sequence of the PxGluClα subunit displayed six mutations that could lead to changes in the amino acid residues. CONCLUSION High suitability related to tenvermectin A resistance was observed in RS, and it was found that the developmental stages of RS were significantly shortened and the survival rate of females was reduced. In addition, the mechanism of resistance to tenvermectin A may be regulated by the glutamate‐gated chloride channel, ATP‐binding cassette transporter, and MFO. In general, the study of resistance and biochemical mechanisms can provide beneficial and rational information for the management of resistance in P. xylostella.Keywords
This publication has 29 references indexed in Scilit:
- Biochemical Mechanism of Chlorantraniliprole Resistance in the Diamondback Moth, Plutella xylostella LinnaeusJournal of Integrative Agriculture, 2014
- Two cytochrome P450 genes are involved in imidacloprid resistance in field populations of the whitefly, Bemisia tabaci, in ChinaPesticide Biochemistry and Physiology, 2013
- Fitness costs associated with insecticide resistancePest Management Science, 2012
- Estimating the Economic Cost of One of the World's Major Insect Pests, Plutella xylostella (Lepidoptera: Plutellidae): Just How Long Is a Piece of String?Journal of Economic Entomology, 2012
- A point mutation in a glutamate‐gated chloride channel confers abamectin resistance in the two‐spotted spider mite, Tetranychus urticae KochInsect Molecular Biology, 2010
- Biochemical analysis and synergistic suppression of indoxacarb resistance in Plutella xylostella L.Journal of Asia-Pacific Entomology, 2010
- Characterisation of abamectin resistance in a field‐evolved multiresistant population of Plutella xylostellaPest Management Science, 2009
- RNA interference-mediated knockdown of a cytochrome P450, CYP6BG1, from the diamondback moth, Plutella xylostella, reduces larval resistance to permethrinInsect Biochemistry and Molecular Biology, 2009
- Monitoring of Diamondback Moth (Lepidoptera: Plutellidae) Resistance to Spinosad, Indoxacarb, and Emamectin BenzoateJournal of Economic Entomology, 2006
- Biology, Ecology, and Management of the Diamondback MothAnnual Review of Entomology, 1993