Electrophoresis of human blood plasma

Abstract

Electrophoretic analysis of human blood serum shows that it contains, in addition to the albumin, 3 globulins, corresponding to those found by Tiselius (Biochem. J. 31, 1464, 1937) in horse serum, and called [alpha], [beta] and [gamma] globulin respectively. Plasma contains one more component, fibrinogen, the boundary of which on migration appears between the [beta] and [gamma] boundaries. The fibrinogen is a cataphoretically well-defined protein with isoelectric point at about pH 5.4. The approx. isoelectric points of globulins a, [beta] and [gamma] are at pH 4.8, 5.2 and 6.4 respectively. The isoelectric point of globulin free serum albumin, isolated by electrophoresis, is at pH 4.64. The electrophoresis technique employed has been descr. by Tiselius (Trans. Faraday Soc. 33, 524, 1937).