A rapid method for the preparation of relatively pure metabolically competent synaptosomes from rat brain
- 15 November 1978
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 176 (2), 365-370
- https://doi.org/10.1042/bj1760365
Abstract
A rapid (< 2h) method is described for the preparation of synaptosomes from rat brain by using a discontinuous Ficoll/sucrose gradient by a flotation technique. These synaptosomes are metabolically active and minimally (< 5%) contaminated with free mitochondria as judged by marker-enzyme assays and EM.This publication has 20 references indexed in Scilit:
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