A PARACRYSTALLINE INCLUSION IN NEUROSPORA CRASSA
Open Access
- 1 October 1971
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 51 (1), 249-264
- https://doi.org/10.1083/jcb.51.1.249
Abstract
A paracrystal indistinguishable from the one which occurs in the mitochondrial mutant abnormal-1 can be induced in wild-type Neurospora crassa after growth in either ethidium or euflavine. This paracrystal has been isolated and partially characterized. It appears to be composed of a single polypeptide (mol wt 68,000) which can be reversibly crystallized and dissociated by changes in the pH and ionic strength. When aggregated, the polypeptide forms oligomers which are arranged end-to-end into fibers. During the characterization of the polypeptide, it was found that the polypeptide's electrophoretic and immunological properties could be used as assays. Using these methods it was found that the polypeptide normally accumulates in a soluble form in the cytoplasm of wild-type Neurospora at the end of the log-phase of growth.Keywords
This publication has 24 references indexed in Scilit:
- Ultrastructure of striated inclusions in NeurosporaJournal of Ultrastructure Research, 1970
- Mitochondria-associated ribonucleic acid of the HeLa cell. Effect of ethidium bromide on the synthesis of ribosomal and 4S ribonucleic acidBiochemistry, 1969
- Mitochondrial-associated 4 s RNA synthesis inhibition by ethidium bromideJournal of Molecular Biology, 1969
- Selective inhibition of the synthesis of mitochondria-associated RNA by ethidium bromideJournal of Molecular Biology, 1969
- BIOGENESIS OF MITOCHONDRIAThe Journal of cell biology, 1969
- DNA biosynthesis in mitochondria: Differential inhibition of mitochondrial and nuclear DNA polymerases by the mutagenic dyes ethidium bromide and acriflavinBiochemical and Biophysical Research Communications, 1969
- RNA Synthesis in Yeast Mitochondria: a Derepressible ActivityNature, 1968
- Ethidium bromide induced mutation of yeast mitochondria: Complete transformation of cells into respiratory deficient non-chromosomal “petites”Biochemical and Biophysical Research Communications, 1968
- A CYTOPLASMIC CHARACTER IN NEUROSPORA CRASSA The Journal of cell biology, 1965
- Electrophoretic separation of the proteins of ribosome subunits and of encephalomyocarditis virusJournal of Molecular Biology, 1964