A bovine acellular scaffold for vocal fold reconstruction in a rat model
- 22 January 2009
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part A
- Vol. 92A (1), 18-32
- https://doi.org/10.1002/jbm.a.32279
Abstract
With a rat model of vocal fold injury, this study examined the in vivo host response to an acellular xenogeneic scaffold derived from the bovine vocal fold lamina propria, and the potential of the scaffold for constructive tissue remodeling. Bilateral wounds were created in the posterior vocal folds of 20 rats, and bovine acellular scaffolds were implanted into the wounds unilaterally, with the contralateral vocal folds as control. The rats were humanely sacrificed after 3 days, 7 days, 1 month, and 3 months, and the coronal sections of their larynges were examined histologically. Expressions of key matrix proteins including collagen I, collagen III, elastin, fibronectin, hyaluronic acid, and glycosaminoglycans (GAGs) were quantified with digital image analysis. Significant infiltration of host inflammatory cells and host fibroblasts in the scaffold implant was observed in the acute stage of wound repair (3 days and 7 days postsurgery). The mean relative densities of collagen I, collagen III, and GAGs in the implanted vocal folds were significantly higher than those in the control after 3 days, followed by gradual decreases over 3 months. Histological results showed that the scaffolds were apparently degraded by 3 months, with no fibrotic tissue formation or calcification. These preliminary findings suggested that the bovine acellular scaffold could be a potential xenograft for vocal fold regeneration. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010Keywords
This publication has 38 references indexed in Scilit:
- Pore Architecture of a Bovine Acellular Vocal Fold ScaffoldTissue Engineering, Part A, 2008
- Relative Contributions of Collagen and Elastin to Elasticity of the Vocal Fold Under TensionAnnals of Biomedical Engineering, 2007
- A Biodegradable, Acellular Xenogeneic Scaffold for Regeneration of the Vocal Fold Lamina PropriaTissue Engineering, 2007
- Collagen composite hydrogels for vocal fold lamina propria restorationBiomaterials, 2005
- Porcine small intestine submucosa (SIS) is not an acellular collagenous matrix and contains porcine DNA: Possible implications in human implantationJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2005
- Principles of Rat Communication: Quantitative Parameters of Ultrasonic Calls in RatsBehavior Genetics, 2005
- The graft content of hyaluronan is increased during xenograft rejectionXenotransplantation, 2004
- Hyaluronan Levels in Acute Vocal Fold ScarThe Laryngoscope, 2004
- Fibronectin Lacking the ED-B Domain Is a Major Structural Component of Tracheal CartilagePublished by Elsevier BV ,1997
- Hyaluronan in the rat with special reference to the skinActa Physiologica Scandinavica, 1988