Molecular analysis of T cell receptor gamma gene expression in allo‐activated splenic T cells of adult mice
- 1 December 1988
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 18 (12), 1907-1915
- https://doi.org/10.1002/eji.1830181207
Abstract
Northern analysis, hybridization in situ and cDNA sequence analysis have been used to demonstrate that the induction of T cell γ-gene expression is a general occurrence when primary splenic T cells of adult mice are cultured in short-term mixed lymphocyte reactions (MLR). Splenic T cells from nine strains of mice examined in eleven different MLR all showed significant induction of γ-RNA, even when the primary T cell response was to only a three amino acid mismatch in a major histocompatibility complex class I antigen. In MLR examined in detail, the expression is highly enriched for in CD3+ “double-negative” T cells (lacking both CD4 and CD8 expression). A cDNA sequence analysis, constituting the first such analysis of any size of γ-gene transcripts from circulating, peripheral cells of adult mice, revealed transcription to be frequently of productively rearranged genes. These genes display extensive junctional diversity.This publication has 44 references indexed in Scilit:
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