Immunocytochemical analysis of P‐fimbrial structure: localization of minor subunits and the influence of the minor subunit FsoE on the biogenesis of the adhesin

Abstract

Antibodies recognizing the non-adhesive minor P-fimbrial subunit protein E and the P-fimbrial adhesin were used in an immunocytochemical analysis of P-fimbrial structure. It was demonstrated that P-fimbriae of the serotypes F71, F72 and F11 carry their adhesin in a complex with protein E. These complexes are commonly found at the tip of the fimbrial structure. In P-fimbriae of serotype F9, expressed by the uropathogenic Escherichia coli strain 21086 adhesin-protein E complexes are localized at the tips as well as along the shafts of the fimbriae. Protein E of F71 fimbriae (FsoE) plays a catalyzing role in the biogenesis of the adhesin, but has no effect on the eventual localization of the adhesin.