Sensitization of lymphocytes against pooled allogeneic cells. I. Generation of cytotoxicity against autologous human lymphoblastoid cell lines.

Abstract

Lymphocytes sensitized in vitro to a pool of X-irradiated allogeneic normal lymphocytes from 20 individuals develop cytotoxic activity for autologous human lymphoblastoid cells (LCL). Pool sensitized T [thymus-derived] lymphocytes lyse autologous LCL cells but they fail to lyse autologous B[bone marrow-derived]-enriched or T-enriched normal target cells or autologous phytohemagglutinin (PHA) blasts. Stimulation with normal cells of single allogeneic individuals rarely led to development of cytotoxicity against autologous LCL cells. Human Epstein-Barr virus transformed LCL cells express target antigens cross-reactive with allogeneic target antigens expressed on normal cells. Sensitization with a pool of allogeneic cells is an effective means of generating effector cells directed against autologous abnormal cells.