Reassessment of Brain Free Fatty Acid Liberation During Global Ischemia and Its Attenuation by Barbiturate Anesthesia
- 28 February 1983
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 40 (3), 880-884
- https://doi.org/10.1111/j.1471-4159.1983.tb08062.x
Abstract
We previously reported that whole-brain free fatty acids (FFA) rose almost linearly for up to 1 h after decapitation of unanesthetized rats and was significantly attenuated by pentobarbital anesthesia. However, our values for total FFA and arachidonic, stearic, oleic, and palmitic acids were severalfold higher than those obtained by previous investigators. Based upon the suggestion that this may be due to FFAs released from di- and triglycerides in the quantitation of FFAs, we have now analyzed and improved our procedures for TLC separation of FFA and reassessed the accumulation of FFA in whole brain during decapitation ischemia in unanesthetized and pentobarbital-anesthetized rats. FFA levels in whole brain after 0.5 min of ischemia were one-half to one- fourth the levels previously reported after 1 min of ischemia. The rise in FFA between 0.5 and 60 min of ischemia was 9-fold for total FFA, and between 7 and 12-fold for each of the FFAs quantitated. Pentobarbital significantly attenuated the rise of all FFAs with, however, greater effects on oleic and palmitic acids than previously reported.Keywords
This publication has 23 references indexed in Scilit:
- Attenuation of Brain Free Fatty Acid Liberation during Global Ischemia: A Model for Screening Potential Therapies for Efficacy?Journal of Cerebral Blood Flow & Metabolism, 1982
- Phenytoin for brain resuscitation after cardiac arrestCritical Care Medicine, 1981
- Effect of pretreatment with thiopental and phenytoin on postischemic brain damage in rabbitsCritical Care Medicine, 1979
- Thiopental Amelioration of Brain Damage after Global Ischemia in MonkeysAnesthesiology, 1978
- Influence of phenobarbital on changes in the metabolites of the energy reserve of the cerebral cortex following complete ischemiaActa Physiologica Scandinavica, 1978
- RESTITUTION OF CEREBRAL ENERGY STATE, AS WELL AS OF GLYCOLYTIC METABOLITES, CITRIC ACID CYCLE INTERMEDIATES AND ASSOCIATED AMINO ACIDS AFTER 30 MINUTES OF COMPLETE ISCHEMIA IN RATS ANAESTHETIZED WITH NITROUS OXIDE OR PHENOBARBITALJournal of Neurochemistry, 1978
- THE RELEASE OF BRAIN FREE FATTY ACIDS DURING ISCHAEMIA IN ESSENTIAL FATTY ACID‐DEFICIENT RATSJournal of Neurochemistry, 1976
- REGIONAL DISTRIBUTION AND RATE OF PRODUCTION OF FREE FATTY ACIDS IN RAT BRAINJournal of Neurochemistry, 1971
- Effects of ischemia and electroconvulsive shock on free fatty acid pool in the brainBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1970
- Measurement of Maximal Permissible Cerebral Ischemia and a Study of Its Pharmacologic ProlongationJournal of Neurosurgery, 1964