Problems associated with the acid hydrolysis of an impure protein preparation
- 1 January 1946
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 40 (1), 88-96
- https://doi.org/10.1042/bj0400088
Abstract
Impure plant leaf protein was hydrolyzed by 10 ml. of 6.08 N-HCl/g. in 75 hrs. as detd. by max. net production of amide-NH3 plus amino-acid "amino" groups. Organic acids arising from decomposition of impurity and by deamina-tion of amino-acids were removed by prolonged extraction of hydrolysate with ether.Keywords
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