THE EFFECTS OF HEMORRHAGE ON TISSUE METABOLITES

Abstract

A method is described for hemorrhaging rats to experimentally produce cases of acute blood loss and hemorrhagic shock. Rats so hemor-rhaged were frozen in liquid air and analyzed for a variety of tissue components, including the high energy phosphate reservoirs and intermediates of carbohydrate metabolism. Complete depletion of these energy reservoirs was found in some of the liver and kidney analyses. The differences between animals expiring in hemorrhagic shock and those meeting a rapid death with post mortem changes were illustrated.