Electrophoretic Behavior of Some Animal Hemoglobins

Abstract

Hemoglobin from 6 spp. of animals was subjected to paper electrophoresis under standard conditions. All showed migration patterns different from those of the known human hemoglobins. Dog and rabbit hemoglobins moved as homogenous compounds at a rate between hemoglobin A and hemoglobin S. The bulk of rat and alligator hemoglobins moved more rapidly than hemoglobin A and produced a trailing spot which could not be resolved into 2 components. Chicken and turtle hemoglobins behaved as mixtures at pH 8.6 and could be resolved into two components at pH 7.4.