Conformational changes of enzymes upon immobilisation
Top Cited Papers
- 13 March 2013
- journal article
- review article
- Published by Royal Society of Chemistry (RSC) in Chemical Society Reviews
- Vol. 42 (15), 6250-6261
- https://doi.org/10.1039/c3cs35495d
Abstract
Protein conformation plays a crucial role in determining both the catalytic efficiency and the chemo-, regio- and enantioselectivity of enzymes, thus eventually influencing their exploitability in biotechnological applications. Inevitably, immobilisation processes alter the natural molecular environment of enzymes, and quite often affect their catalytic activity through different mechanisms such as reduced accessibility of the substrate to the catalytic active centre, loss of the enzyme dynamic properties and alteration of the conformational integrity of the enzyme. This tutorial review outlines first the most common spectroscopic techniques used for investigating the conformation of immobilized proteins, and then examines how protein loading and polar and hydrophobic/hydrophilic interactions with the carrier affect the structural and dynamic features of enzymes. The nanoscale-level studies in which protein conformational changes, determined either by experimental approaches or by homology modelling, are correlated with the size and shape of the support are also discussed. Altogether, these results should provide useful information on how supports and/or enzymes have to be tailored to improve biocatalyst performance.Keywords
This publication has 56 references indexed in Scilit:
- Immobilization and Photocurrent Activity of a Light-Harvesting Antenna Complex II, LHCII, Isolated from a Plant on ElectrodesACS Macro Letters, 2012
- Enzymes on material surfacesColloids and Surfaces B: Biointerfaces, 2012
- Enhancing the functional properties of thermophilic enzymes by chemical modification and immobilizationEnzyme and Microbial Technology, 2011
- Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of EnzymesAdvanced Synthesis & Catalysis, 2011
- Perspective of Recent Progress in Immobilization of EnzymesACS Catalysis, 2011
- Biomolecule‐Functionalized Nanowires: From Nanosensors to NanocarriersChemphyschem, 2009
- Advances in enzyme immobilisationBiotechnology Letters, 2009
- Understanding enzyme immobilisationChemical Society Reviews, 2008
- Enzyme-semiconductor interactions: Routes from fundamental aspects to photoactive devicesPhysica Status Solidi (b), 2008
- Structural features of thermozymesBiotechnology Advances, 2005