Evidence for Papillomavirus Genus-Specific Antigens and DNA in Laryngeal Papilloma
- 1 January 1981
- journal article
- research article
- Published by S. Karger AG in Intervirology
- Vol. 15 (4), 204-212
- https://doi.org/10.1159/000149233
Abstract
Four juvenile laryngeal papillomas (JLPs) were examined for papillomavirus genus-specific structural antigens and polynucleotide sequences employing reagents derived from bovine papillomavirus type 1 (BPV-1). Three of four JLPs contained intranuclear antigens in the granular layer of differentiating epithelial cells of formalin-fixed, paraffin-embedded papillomas assayed by the peroxidase-antiperoxidase reaction using an antiserum prepared against purified detergent-disrupted BPV-1. Examination of these JLPs for viral sequences indicated two tumor DNA preparations contained polynucleotide sequences which hybridized to an in vitro labeled BPV-1 DNA probe under nonstringent conditions. These sequences were detectable after gel electrophoresis and immobilization of restriction-endonuclease-digested JLP DNAs onto nitrocellulose membranes. Specific hybridization of probe to digested JLP DNA was visualized as bands in autoradiograms coincidental with open circular and linearized papillomavirus DNA. Hybridization was extensively reduced when JLP DNA preparations were prehybridized to an unlabeled papillomavirus DNA heterologous to the probe. These results provide direct evidence for the association of a papillomavirus in JLP.Keywords
This publication has 15 references indexed in Scilit:
- Immunologic Relatedness of Papillomaviruses From Different Species2JNCI Journal of the National Cancer Institute, 1980
- Conserved polynucleotide sequences among the genomes of papillomavirusesJournal of Virology, 1979
- Evidence for antigenic determinants shared by the structural polypeptides of (Shope) rabbit papillomavirus and human papillomavirus type 1Virology, 1978
- Demonstration of two distinct classes of bovine papilloma virusVirology, 1978
- Characterization of two types of human papillomaviruses in lesions of epidermodysplasia verruciformisProceedings of the National Academy of Sciences, 1978
- Papova-virus in human laryngeal papillomasArchives of Oto-Rhino-Laryngology, 1978
- Characterization of a New Type of Human Papillomavirus That Causes Skin WartsJournal of Virology, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Human papillomavirus DNA: physical mapping of the cleavage sites of Bacillus amyloliquefaciens (BamI) and Haemophilus parainfluenzae (HpaII) endonucleases and evidence for partial heterogeneityJournal of Virology, 1977
- Human papilloma viruses (HPV): Characterization of four different isolatesVirology, 1977