Stabilizing effect of amphiphilic excipients on the freeze‐thawing and freeze‐drying of lactate dehydrogenase
- 1 May 1994
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 43 (11), 1102-1107
- https://doi.org/10.1002/bit.260431114
Abstract
The effects of amphiphilic excipients on the inactivation of lactate dehydrogenase (LDH) during freeze-thawing and freeze-drying were studied. Some amphiphilic excipients such as hydroxypropyl-β-cyclodextrin (HP-β-CD), CHAPS, polyethylene glycol (PEG) 3350, and sucrose fatty acid monoester prevented LDH inactivation during freeze-thawing and freeze-drying at a lower concentration than sugars and amino acids. Polyoxyethylene 9 lauryl ether and PEG 400 protected LDH during freeze-thawing but not during freeze-drying. The buffer concentration of the solution to be freeze-dried (10, 50, and 200 mM) affected the stabilizing effect of trehalose, but not that of HP-β-CD. © 1994 John Wiley & Sons, Inc.Keywords
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