Multiplication-Stimulating Activity Stimulates the Multiplication of F9 Embryonal Carcinoma Cells*
- 1 April 1982
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 110 (4), 1231-1237
- https://doi.org/10.1210/endo-110-4-1231
Abstract
The present study was designed to assess the effectiveness of multiplication-stimulating activity (MSA), an insulin-like growth factor, in supporting the growth of F9 cells (an embryonal carcinoma line with properties similar to embryonic stem cells). Under serum-free growth conditions in a medium supplemented with transferrin and fibronectin, MSA is an effective mitogen. At 100 ng/ml, MSA completely replaces the growth requirement for fetal calf serum. Biologically active MSA polypeptides II and III act as potent growth promoters of F9 cells, whereas MSA I appears to be somewhat less effective. Binding studies carried out with [125I]iodo-MSA indicate that F9 cells possess specific receptors for MSA. Scatchard analysis indicates a single class of MSA receptors with a Ka of 8.2 × 109 M-1; about 55,000 MSA molecules can bind per F9 cell. Insulinlike growth factors I and II both compete for MSA binding, whereas insulin does not compete. Since insulin is an effective promoter of F9 cell growth, these results indicate that the mitogenic action of insulin toward F9 cells is not mediated through MSA receptors. It is apparent from these results that MSA is capable of serving as an early embryonic growth factor.Keywords
This publication has 19 references indexed in Scilit:
- The cell surface antigens of mouse embryonal carcinoma cellsBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1978
- Purified Human Somatomedin A and Rat Multiplication Stimulating ActivityEuropean Journal of Biochemistry, 1978
- The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cellsDevelopmental Biology, 1977
- Effects of Peptide Anabolic Hormones on Growth of Myoblasts in CultureEndocrinology, 1977
- Characterization of the binding of multiplication-stimulating activity to a receptor for growth polypeptides in chick embryo fibroblasts.Journal of Biological Chemistry, 1977
- Identification of a Receptor for Somatomedin-Like Polypeptides in Human Fibroblasts*Journal of Clinical Endocrinology & Metabolism, 1977
- Stimulation of DNA synthesis, cell multiplication, and ornithine decarboxylase in 3T3 cells by multiplication stimulating activity (MSA)Journal of Cellular Physiology, 1976
- Polypeptides with nonsuppressible insulin-like and cell-growth promoting activities in human serum: isolation, chemical characterization, and some biological properties of forms I and II.Proceedings of the National Academy of Sciences, 1976
- ULTRASTRUCTURE OF PRIMORDIAL GERMINAL CELLS OF FETAL TESTES + OF EMBRYONAL CARCINOMA CELLS OF MICE1964
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951