High-performance liquid chromatography of pyruvic and alpha-ketoglutaric acids and its application to urine samples.
- 1 May 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 23 (5), 802-805
- https://doi.org/10.1093/clinchem/23.5.802
Abstract
Pyruvic and alpha-ketoglutaric acids were quantitatively and rapidly converted to quinoxalones by a new method, to enhance their detectability in the ultraviolet. The quinoxalones were separated by reversed-phase chromatography with use of 10 micronm particles packed in a 30-cm (length) column. An aqueous solution of ammonium acetate and methanol was used for linear gradient elution. The urinary excretion of pyruvic and alpha-ketoglutaric acids by normal individuals, diabetic patients, and patients with renal dysfunction was determined.This publication has 1 reference indexed in Scilit:
- A combined enzymatic method for measuring α-oxoglutarate and pyruvate in blood and urineClinica Chimica Acta; International Journal of Clinical Chemistry, 1961