The proportions of cell content, nitrogen, nitrate-nitrogen and water-soluble carbohydrate in three grasses in the early stages of regrowth after defoliation with and without applied nitrogen

Abstract

SUMMARY: The regrowth of Aberystwyth S. 22 Italian ryegrass, S. 24 perennial ryegrass and S. 37 cocksfoot was studied in field swards with and without applied nitrogen during the 21 days following a cut or grazing, there being a total of five periods of study at different tunes of year and in different years.The proportion of cell content in the harvested herbage, as measured by neutral detergent, increased by nearly 9 percentage units from day 3 to day 18 of regrowth. The proportion of cell content as measured by acid pepsin also increased during regrowth, but to a lesser extent. The proportion of cell content in leaf blades of two age categories, as measured by neutral detergent, also increased during regrowth. The above increases in proportion of cell content were found in all three grasses. The increases were associated with increases in the proportion of crude protein in dry matter, which were only partially offset by reductions in the proportion of water-soluble carbohydrate. The broad pattern of change in N and nitrate-N content of Italian ryegrass herbage during regrowth in spring with different amounts of N applied, noted in earlier experiments, was confirmed and found to apply to perennial ryegrass and cocksfoot also. During a September-October period of study there was little sign of decline in nitrate-N content within the 21 days where N had been applied. Younger leaf blades had a rather lower nitrate-N content and a slightly higher total N content, on average, than, older blades. The application of N increased the proportion of crude protein more than it reduced the proportion of water-soluble carbohydrate and it increased the proportion of cell content. The increase in proportion of cell content was sufficient to explain the positive effect of N application on digestibility. The average proportion of total herbage N which was found in the cell wall fraction was about 12%.There appeared to be a relationship between chemical composition and dry-matter content. The chlorophyll content of leaf blades increased during regrowth and was increased by N application. The experimental results on balance, particularly those for proportion of cell content, suggest an improvement in nutritive value of grass during regrowth up to about 3 weeks in contrast to the well-recognized decline in nutritive value where regrowth proceeds beyond about 3 weeks.