Increase of gap junctions between pancreatic B-cells during stimulation of insulin secretion.
Open Access
- 1 August 1979
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 82 (2), 441-448
- https://doi.org/10.1083/jcb.82.2.441
Abstract
The development of gap junctions between pancreatic B-cells was quantitatively assessed in freeze-fracture replicas of isolated rat islets under different conditions of insulin secretion. The results show that in resting B-cells, gap junctions are small and scarce but that these junctions increase when insulin secretion is stimulated. Both a short (90 min) stimulation by glucose in vitro and a prolonged (2.5 d) stimulation by glibenclamide in vivo raise the number of gap junctions; in addition, the glibenclamide stimulation causes an increase in the size of individual gap junctions. As a consequence, the total area occupied by gap junctions on the B-cell membrane and the ratio of this area to the cell volume were found significantly increased in the latter condition. The slight increase of these values observed after the glucose stimulation did not reach significance. These data indicate a change of gap junctions during the secretory activity of the pancreatic B-cells. The possibility that the coupling of the cells is affected by the treatment is discussed.This publication has 34 references indexed in Scilit:
- Quantitative analysis of low-resistance junctions between cultured cells and correlation with gap-junctional areas.The Journal of cell biology, 1978
- Intercellular Communication in Insect Development Is Hormonally ControlledScience, 1978
- Intercellular junctions of oral epitheliumJournal of Ultrastructure Research, 1977
- Gap junctions. Structural changes after uncoupling procedures.The Journal of cell biology, 1977
- Electrophysiological evidence for coupling between β cells of pancreatic isletsNature, 1976
- Hormonal regulation of gap junction differentiation.The Journal of cell biology, 1976
- FUNCTIONAL SUBDIVISION OF ISLETS OF LANGERHANS AND POSSIBLE ROLE OF D CELLSThe Lancet, 1975
- A morphological basis for intercellular communication between alpha- and beta-cells in the endocrine pancreas.JCI Insight, 1975
- Somatostatin: Hypothalamic Inhibitor of the Endocrine PancreasScience, 1974
- Structural coupling between pancreatic islet cellsCellular and Molecular Life Sciences, 1973