Efficient pH-independent sequence-specific DNA binding by pseudoisocytosine-containing bis-PNA
- 1 January 1995
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 23 (2), 217-222
- https://doi.org/10.1093/nar/23.2.217
Abstract
The synthesis and DNA binding properties of bis-PNA (peptide nucleic acid) are reported. Two PNA segments each of seven nucleobases in length were connected in a continuous synthesis via a flexible linker composed of three 8-amino-3,6-dioxaoctanoic acid units. The sequence of the first strand was TCTCTTT (C- to N-terminal), while the second strand was TTTCTCT or TTTJTJT, where J is pseudoisocytosine. These bis-PNAs form triple-stranded complexes of somewhat higher thermal stability than monomeric PNA with complementary oligonucleotides and the thermal melting transition shows very little hysteresis. When the J base is placed in the strand parallel to the DNA complement ('Hoogsteen strand'), the DNA binding was pH independent. The bis-PNAs were also superior to monomeric PNAs for targeting double-stranded DNA by strand invasion.Keywords
This publication has 10 references indexed in Scilit:
- PNA hybridizes to complementary oligonucleotides obeying the Watson–Crick hydrogen-bonding rulesNature, 1993
- 7,8-Dihydro-8-oxoadenine as a replacement for cytosine in the third strand of triple helixes. Triplex formation without hypochromicityBiochemistry, 1993
- DNA unwinding upon strand-displacement binding of a thymine-substituted polyamide to double-stranded DNA.Proceedings of the National Academy of Sciences, 1993
- Recognition of a guanine-cytosine base pair by 8-oxoadenineBiochemistry, 1992
- Oligonucleotide-mediated triple helix formation using an N3-protonated deoxycytidine analog exhibiting pH-independent binding within the physiological range.Proceedings of the National Academy of Sciences of the United States of America, 1992
- Uranyl photofootprinting of triple helical DNANucleic Acids Research, 1992
- Sequence-Selective Recognition of DNA by Strand Displacement with a Thymine-Substituted PolyamideScience, 1991
- Sequence-Specific Cleavage of Double Helical DNA by Triple Helix FormationScience, 1987
- Models of triple-stranded polynucleotides with optimised stereochemistryNucleic Acids Research, 1976
- Synthesis of 5-carboxymethyluridine. Nucleoside from transfer ribonucleic acidBiochemistry, 1970