Application of Plackett-Burman design and response surface methodology to achieve exponential growth for aggregated shipworm bacterium
- 2 February 2004
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 85 (6), 666-675
- https://doi.org/10.1002/bit.10880
Abstract
Here we report the successful implementation of the Plackett‐Burman multifactorial design to screen the limiting components for growth and subsequent use of the response surface methodology (RSM) to design a medium that supported exponential growth of the aggregated morphology of the shipworm bacterium, Teredinobacter turnirae. The results obtained with the help of Plackett‐Burman design indicated limitations of three components in the growth medium, MnCl2.4H2O, Na2CO3, and K2HPO4. The concentrations of these three components were further optimized using RSM. By increasing the concentrations of the above‐mentioned components by 4‐fold, 12‐fold, and 12‐fold, respectively, it became possible to achieve exponential growth of the culture.Keywords
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