miR-203 Inhibits Cell Proliferation and Migration of Lung Cancer Cells by Targeting PKCα
Open Access
- 10 September 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (9), e73985
- https://doi.org/10.1371/journal.pone.0073985
Abstract
PKCα (protein kinase C alpha, PRKCA) is an important protein involved in several steps of signaling pathways in lung cancer, and microRNAs (miRNAs) have also been shown to participate in lung carcinogenesis. However, it is not clear how PKCα and miRNAs are correlated in the disease. In this report, we aimed to identify novel miRNAs that target PKCα and to study their biological function. Using bioinformatics analysis, we predicted one novel candidate, miR-203, and found differential expression patterns of miR-203 and PKCα in human lung cancer tissues. Moreover, we experimentally validated miR-203 as a direct regulator of PKCα. Finally, we demonstrated that the targeting of PKCα by miR-203 played a critical role in regulating cell proliferation, apoptosis and migration in lung cancer cells. In summary, this study identifies a novel miRNA that targets PKCα and illustrates that the downregulation of PKCα by miR-203 modulates biological processes in lung cancer cells.Keywords
This publication has 41 references indexed in Scilit:
- RETRACTED ARTICLE: EGFR and MET receptor tyrosine kinase–altered microRNA expression induces tumorigenesis and gefitinib resistance in lung cancersNature Medicine, 2011
- Protein Kinase Cα Promotes Cell Migration through a PDZ-Dependent Interaction with its Novel Substrate Discs Large Homolog 1 (DLG1)Journal of Biological Chemistry, 2011
- Tumor-suppressive miR-34a induces senescence-like growth arrest through modulation of the E2F pathway in human colon cancer cellsProceedings of the National Academy of Sciences, 2007
- MicroRNA-34a functions as a potential tumor suppressor by inducing apoptosis in neuroblastoma cellsOncogene, 2007
- MicroRNA Signatures in Human CancersNature Reviews Cancer, 2006
- Oncomirs — microRNAs with a role in cancerNature Reviews Cancer, 2006
- A microRNA polycistron as a potential human oncogeneNature, 2005
- Combinatorial microRNA target predictionsNature Genetics, 2005
- The functions of animal microRNAsNature, 2004
- Prediction of Mammalian MicroRNA TargetsCell, 2003