Formation of the First Peptide Bond: The Structure of EF-P Bound to the 70 S Ribosome
- 21 August 2009
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 325 (5943), 966-970
- https://doi.org/10.1126/science.1175800
Abstract
Protein Synthesis Initiation Complex: The final step in the initiation phase of protein synthesis is the formation of the first peptide bond, which requires initiator transfer RNA (tRNA) to be bound at the ribosomal P site. Elongation factor P (EF-P) is a protein conserved in all eubacteria that stimulates this initial bond formation. Insight into how this is achieved comes from a structure of Thermus thermophilus 70 S ribosome bound to EF-P, initiator tRNA, and a short piece of messenger RNA presented by Blaha et al. (p. 966 ). EF-P binds between the P and E sites and facilitates proper positioning of initiator tRNA in the P site. A similar mechanism is likely to apply to structurally homologous initiation factors in archea and eukarya.Keywords
This publication has 40 references indexed in Scilit:
- Hypusine-containing protein eIF5A promotes translation elongationNature, 2009
- Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosomeNature Structural & Molecular Biology, 2009
- Following movement of the L1 stalk between three functional states in single ribosomesProceedings of the National Academy of Sciences of the United States of America, 2009
- Is there a role for eIF5A in translation?Amino Acids, 2007
- Structural Basis for Interaction of the Ribosome with the Switch Regions of GTP-Bound Elongation FactorsMolecular Cell, 2007
- Structure of the 70 S Ribosome Complexed with mRNA and tRNAScience, 2006
- A Protein Component at the Heart of an RNA Machine: The Importance of Protein L27 for the Function of the Bacterial RibosomeMolecular Cell, 2005
- The Cryo-EM Structure of a Translation Initiation Complex from Escherichia coliCell, 2005
- Initiation of Protein Synthesis in BacteriaMicrobiology and Molecular Biology Reviews, 2005
- Feedback regulation of rRNA synthesis in Escherichia coliJournal of Molecular Biology, 1987