Increasing relative prevalence of HSV-2 infection among men with genital ulcers from a mining community in South Africa
Open Access
- 1 June 2003
- journal article
- research article
- Published by BMJ in Sexually Transmitted Infections
- Vol. 79 (3), 202-207
- https://doi.org/10.1136/sti.79.3.202
Abstract
Objectives: To determine the aetiology of genital ulcer disease (GUD) and its association with HIV infection in the mining community of Carletonville, South Africa, from two cross sectional surveys of consecutive men presenting with genital lesions during October 1993 to January 1994 and July to November 1998. Methods: A multiplex polymerase chain reaction (M-PCR) assay combined with amplicon detection was used to identify DNA specific sequences of Treponema pallidum, herpes simplex virus (HSV), and Haemophilus ducreyi. A real time PCR assay was used to differentiate between HSV-1 and HSV-2. Results: M-PCR detected T pallidum, HSV, and H ducreyi in 10.3%, 17.2%, and 69.4% of 232 GUD patients during 1993–4 and in 12.4%, 36.0%, and 50.5% of 186 GUD patients in 1998. The proportion of patients with more than one agent increased significantly from 7.3% (17/232) in 1993–4 to 16.7% (31/186) in 1998 (p v 6.7%, p 0.09). Conclusions: Based on two cross sectional surveys, 4 years apart, chancroid remained the leading cause of GUD in men who presented at the STD clinic with genital ulcers in the mining community of Carletonville, South Africa. The relative prevalence of primary syphilis has remained low. However, HSV-2 has emerged as a more significant cause of GUD and the proportion of GUD patients infected with more than one agent also increased significantly. HSV-2 DNA was detected in a significantly higher proportion of ulcer specimens from HIV positive patients than from HIV negative patients. No association was found between HIV infection status and the relative prevalence of chancroid or syphilis.Keywords
This publication has 31 references indexed in Scilit:
- Association of CD4 Cell Depletion and Elevated Blood and Seminal Plasma Human Immunodeficiency Virus Type 1 (HIV‐1) RNA Concentrations with Genital Ulcer Disease in HIV‐1‐Infected Men in MalawiThe Journal of Infectious Diseases, 1998
- Sexually transmitted diseases in South Africa.Sexually Transmitted Infections, 1996
- The High Prevalence of Genital Herpes Among Patients With Genital Ulcer Disease in UgandaSexually Transmitted Diseases, 1995
- Laboratory techniques in the diagnosis of herpes simplex infection.Sexually Transmitted Infections, 1993
- Cofactors in Male-Female Sexual Transmission of Human Immunodeficiency Virus Type 1The Journal of Infectious Diseases, 1991
- Genital ulcers and transmission of HIV among couples in ZimbabweAIDS, 1989
- Genital ulceration as a risk factor for human immunodeficiency virus infectionAIDS, 1988
- Heterosexual transmission of HIVAIDS, 1988
- DNA Sequence and Genetic Content of the HindIII l Region in the Short Unique Component of the Herpes Simplex Virus Type 2 Genome: Identification of the Gene Encoding Glycoprotein G, and Evolutionary ComparisonsJournal of General Virology, 1987
- Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1Journal of Molecular Biology, 1985