Insecticide Detection through Protein Engineering of Nippostrongylus brasiliensis Acetylcholinesterase B
- 19 August 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 77 (18), 5823-5830
- https://doi.org/10.1021/ac050383p
Abstract
The sensitivity of acetylcholinesterase (AChE) biosensors for insecticide detection could be increased substantially by engineering AChE B of Nippostrongylus brasiliensis. The introduction of 10 single and 4 double mutations into the AChE peptide chain led to an increase in sensitivity to 10 of the 11 insecticides tested. The combination of three mutants with the wild-type enzyme in a multienzyme biosensor array enabled the detection of 11 out of the 14 most important organophosphates and carbamates at concentrations below 10 μg/kg, the maximum residue limit of infant food. The detection limit for pirimiphos methyl could be reduced from 10 μg/L to a value as low as 1 ng/L (3.5 × 10-12 mol/L). The newly created biosensors exhibited an extraordinary high storage stability. There was no loss of sensitivity of N. brasiliensis AChE B, immobilized on screen-printed, disposable electrodes, even after 17-month storage at room temperature.Keywords
This publication has 43 references indexed in Scilit:
- FURTHER ASSESSMENT OF ANIN VITROSCREEN THAT MAY HELP IDENTIFY ORGANOPHOSPHORUS PESTICIDES THAT ARE MORE ACUTELY TOXIC TO THE YOUNGJournal of Toxicology and Environmental Health, Part A, 2004
- New principle of direct real-time monitoring of the interaction of cholinesterase and its inhibitors by piezolectric biosensorBiosensors and Bioelectronics, 2003
- Development, validation, and application of an acetylcholinesterase-biosensor test for the direct detection of insecticide residues in infant foodBiosensors and Bioelectronics, 2002
- Pesticides in dietary foods for infants and young children.Archives of Disease in Childhood, 1999
- Determination of carbamate residues in crop samples by cholinesterase-based biosensors and chromatographic techniquesAnalytica Chimica Acta, 1998
- The ADI as a basis to establish standards for pesticide residues in food products for infants and childrenFood Additives & Contaminants, 1998
- Potentiometric biosensors for cholinesterase inhibitor analysis based on mediatorless bioelectrocatalysisBiosensors and Bioelectronics, 1996
- Determination of organophosphorous and carbamate insecticides by flow injection analysisAnalytical Biochemistry, 1992
- Sensitive detection of pesticides using amperometric sensors based on cobalt phthalocyanine-modified composite electrodes and immobilized cholinesterasesBiosensors and Bioelectronics, 1992
- Determination of organophosphate and carbamate pesticides using a cobalt phthalocyanine-modified carbon paste electrode and a cholinesterase enzyme membraneAnalytica Chimica Acta, 1991