Phosphotriesters in rat liver deoxyribonucleic acid after the administration of the carcinogen NN-dimethylnitrosamine in vivo
- 1 March 1975
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 145 (3), 475-482
- https://doi.org/10.1042/bj1450475
Abstract
After treatment with NN-di[14C]methylnitrosamine, samples of DNA were isolated from rat livers by a conventional phenol procedure and examined for the presence of phosphotriesters. A method of capable of detecting relatively small amounts of 14C-labelled phosphotriesters was developed and used to establish that these products account for 10-12% of the total methylation pattern found after treatment with this agent in vitro. The significance of the presence of phosphotriesters in DNA is discussed.Keywords
This publication has 17 references indexed in Scilit:
- The molecular basis for biological inactivation of nucleic acids. The action of methylating agents on the ribonucleic acid-containing bacteriophage R17Biochemical Journal, 1974
- The reaction of alkylating agents with bacteriophage R17. Biological effects of phosphotriester formationBiochemical Journal, 1974
- Reaction of N-methyl-N-nitrosourea (MNUA) with 32P-labelled DNA: evidence for formation of phosphotriestersChemico-Biological Interactions, 1973
- Alkylation of Phosphates and Stability of Phosphate Triesters in DNAEuropean Journal of Biochemistry, 1972
- Differences in the patterns of methylation in rat liver ribosomal ribonucleic acid after reaction in vivo with methyl methanesulphonate and NN-dimethylnitrosamineBiochemical Journal, 1972
- Methylated polydeoxyribocytidylic acid templates for RNA polymeraseBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Ethylation of polyadenylic acidBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- Nucleic AcidsAnnual Review of Biochemistry, 1955
- THE CHROMATOGRAPHIC IDENTIFICATION OF SOME BIOLOGICALLY IMPORTANT PHOSPHATE ESTERSJournal of Biological Chemistry, 1951
- The reaction of nucleic acids with mustard gas1948