Complement Fixation on Cell Surfaces by 19 S and 7 S Antibodies
- 22 October 1965
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 150 (3695), 505-506
- https://doi.org/10.1126/science.150.3695.505
Abstract
The mechanism of complement fixation on cell surfaces by whole antiserums, and by 19S and 7S fractions has been studied with a new comple-ment-fixation test. This test is based on the fixation and transfer of the activated first component of complement (Ć1a). We have concluded that a single molecule of 19S antibody in combination with antigen at the cell surface is sufficient to bind one molecule of Ć1a. For 7S antibodies at least two molecules in close proximity at the cell surface are required to fix one molecule of Ć1a.This publication has 4 references indexed in Scilit:
- Hemolysin Titration Based on Fixation of the Activated First Component of Complement: Evidence That One Molecule of Hemolysin Suffices to Sensitize an ErythrocyteThe Journal of Immunology, 1965
- Chromatographic Separation of the First Component of Complement and Its Assay on a Molecular BasisThe Journal of Immunology, 1963
- Effects of Low Ionic Strength on Immune HemolysisThe Journal of Immunology, 1963
- The Relation between Hemolysin Concentration and Hemolytic Rate as Measured with Chromium51 Labeled CellsThe Journal of Infectious Diseases, 1958