Regulation of development in Dictyostelium discoideum. IV. Effects of ions on the rate of differentiation and cellular response to cyclic AMP.

Abstract

The effects of ionic environment on both the intrinsic rate of differentiation and the response to exogenous cAMP in D. discoideum were examined. K+ specifically inhibits the rate of early development when present at concentrations > 15 mM. Na+ does not inhibit at concentrations up to 25 mM, and can partially overcome K+ inhibition. The maximum rate of development also depends upon the presence of adequate levels of extracellular Ca2+. The effect of exogenous cAMP on the rate of development depends on the ionic environment. Stimulation is observed only when the rate of development is inhibited by the absence of Ca2+, and/or the presence of high concentrations of K+. Under optimal ionic conditions, the only effect of exogenous cAMP on early development is a specific inhibition. The implications of these results for current models of early developmental regulation are discussed.