Thymus-homing peripheral dendritic cells constitute two of the three major subsets of dendritic cells in the steady-state thymus
Open Access
- 9 March 2009
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 206 (3), 607-622
- https://doi.org/10.1084/jem.20082232
Abstract
Many dendritic cells (DCs) in the normal mouse thymus are generated intrathymically from common T cell/DC progenitors. However, our previous work suggested that at least 50% of thymic DCs originate independently of these progenitors. We now formally demonstrate by parabiotic, adoptive transfer, and developmental studies that two of the three major subsets of thymic DCs originate extrathymically and continually migrate to the thymus, where they occupy a finite number of microenvironmental niches. The thymus-homing DCs consisted of immature plasmacytoid DCs (pDCs) and the signal regulatory protein α–positive (Sirpα+) CD11b+ CD8α− subset of conventional DCs (cDCs), both of which could take up and transport circulating antigen to the thymus. The cDCs of intrathymic origin were mostly Sirpα− CD11b− CD8αhi cells. Upon arrival in the thymus, the migrant pDCs enlarged and up-regulated CD11c, major histocompatibility complex II (MHC II), and CD8α, but maintained their plasmacytoid morphology. In contrast, the migrant cDCs proliferated extensively, up-regulated CD11c, MHC II, and CD86, and expressed dendritic processes. The possible functional implications of these findings are discussed.Keywords
This publication has 71 references indexed in Scilit:
- Dendritic cells in the thymus contribute to T-regulatory cell inductionProceedings of the National Academy of Sciences of the United States of America, 2008
- In Vitro-Generated Antigen-Specific CD4+CD25+Foxp3+Regulatory T Cells Control the Severity of Herpes Simplex Virus-Induced Ocular Immunoinflammatory LesionsJournal of Virology, 2008
- Nonself-Antigens Are the Cognate Specificities of Foxp3+ Regulatory T CellsImmunity, 2007
- Origin of dendritic cells in peripheral lymphoid organs of miceNature Immunology, 2007
- Plasmacytoid dendritic cells prime IL-10–producing T regulatory cells by inducible costimulator ligandThe Journal of Experimental Medicine, 2007
- Intrasplenic steady-state dendritic cell precursors that are distinct from monocytesNature Immunology, 2006
- Hassall's corpuscles instruct dendritic cells to induce CD4+CD25+ regulatory T cells in human thymusNature, 2005
- Promiscuous gene expression in thymic epithelial cells is regulated at multiple levelsThe Journal of Experimental Medicine, 2005
- Mouse CD11c+ B220+ Gr1+plasmacytoid dendritic cells develop independently of the T-cell lineageBlood, 2002
- Studies of thymocytopoiesis in rats and mice. I. Kinetics of appearance of thymocytes using a direct intrathymic adoptive transfer assay for thymocyte precursors.The Journal of Experimental Medicine, 1986