Brain Cells in Culture: Morphological Transformation by a Protein
- 5 July 1974
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 185 (4145), 63-66
- https://doi.org/10.1126/science.185.4145.63
Abstract
One type of elmbryonic rat brain cell having an epithelioid morphology in the monolayer culture can be transformed by brain extract into cells having extensive processes resembling mature astrocytes. The transforming factor is a protein with a molecular weight of 350,000. A partially purified sample showed that it is active at a concentration as low as 1 x 10-8M. The transforming actvity is high in adult brains but low in embryonic brains and tumors of the nervous systems.Keywords
This publication has 5 references indexed in Scilit:
- Morphological and Biochemical Alterations in Foetal Rat Brain Cells cultured in the Presence of Monobutyryl Cyclic AMPNature, 1973
- Reconstruction of brain tissue from cell suspensions: I. Aggregation patterns of cells dissociated from different regions of the developing brainDevelopmental Biology, 1972
- Dissociation, fractionation, and culture of embryonic brain cellsBrain Research, 1969
- Propagation of human fetal spongioblasts and astrocytes in dispersed cell culturesExperimental Cell Research, 1965
- An improved nutrient solution for diploid Chinese hamster and human cell linesExperimental Cell Research, 1963