Synthesis and biological evaluation of human preproenkephalin (100-111) and its analogs*
- 1 February 1989
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 33 (2), 77-81
- https://doi.org/10.1111/j.1399-3011.1989.tb00190.x
Abstract
The dodecapeptide sequence, Tyr-Gly-Gly-Phe-Met-Lys-Arg-Tyr-Gly-Gly-Phe-Met (BI), which is totally conserved in the primary structures of human, bovine, rat, and toad preproenkephalins, has been synthesized by the solid-phase method. Coupling reactions were achieved by using symmetrical anhydrides of tert.-butyloxycarbonylamino acids preformed with N-tert.-butyl,N′-methylcarbodiimide. 6-Arg and 7-Lys analogs have also been obtained. The peptides show opiate activity in both GPI and MVD assay, and possess antinociceptive properties as estimated by the hot-plate test in mice when applied intracisternally.Keywords
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