Isolation and characterization of an arsenate-reducing bacterium and its application for arsenic extraction from contaminated soil
Open Access
- 1 January 2012
- journal article
- Published by Oxford University Press (OUP) in Journal of Industrial Microbiology & Biotechnology
- Vol. 39 (1), 37-44
- https://doi.org/10.1007/s10295-011-0996-6
Abstract
A Gram-negative anaerobic bacterium, Citrobacter sp. NC-1, was isolated from soil contaminated with arsenic at levels as high as 5,000 mg As kg−1. Strain NC-1 completely reduced 20 mM arsenate within 24 h and exhibited arsenate-reducing activity at concentrations as high as 60 mM. These results indicate that strain NC-1 is superior to other dissimilatory arsenate-reducing bacteria with respect to arsenate reduction, particularly at high concentrations. Strain NC-1 was also able to effectively extract arsenic from contaminated soils via the reduction of solid-phase arsenate to arsenite, which is much less adsorptive than arsenate. To characterize the reductase systems in strain NC-1, arsenate and nitrate reduction activities were investigated using washed-cell suspensions and crude cell extracts from cells grown on arsenate or nitrate. These reductase activities were induced individually by the two electron acceptors. This may be advantageous during bioremediation processes in which both contaminants are present.Keywords
This publication has 32 references indexed in Scilit:
- Slurry bioreactor modeling using a dissimilatory arsenate-reducing bacterium for remediation of arsenic-contaminated soilJournal of Bioscience and Bioengineering, 2009
- Arsenic extraction from solid phase using a dissimilatory arsenate-reducing bacteriumJournal of Bioscience and Bioengineering, 2005
- The Ecology of ArsenicScience, 2003
- Properties of the periplasmic nitrate reductases fromParacoccus pantotrophusandEscherichia coliafter growth in tungsten-supplemented mediaFEMS Microbiology Letters, 2003
- Dissimilatory arsenate reductase activity and arsenate-respiring bacteria in bovine rumen fluid, hamster feces, and the termite hindgutFEMS Microbiology Ecology, 2002
- Homology of Escherichia coli R773 arsA , arsB , and arsC Genes in Arsenic-Resistant Bacteria Isolated from Raw Sewage and Arsenic-Enriched Creek WatersApplied and Environmental Microbiology, 2002
- India's Spreading Health Crisis Draws Global Arsenic ExpertsScience, 1996
- Precipitation of arsenic during bacterial sulfate reductionGeomicrobiology Journal, 1995
- Effect of tungsten on nitrate and nitrite reductases in Azospirillum brasilense Sp7Canadian Journal of Microbiology, 1991
- Relationship between adsorption of arsenic(III) and boron by soil and soil propertiesEnvironmental Science & Technology, 1987