Relationship Between the Age of Human Erythrocytes and Their Osmotic Resistance: A Basis for Separating Young and Old Erythrocytes12
- 1 November 1958
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 37 (11), 1542-1548
- https://doi.org/10.1172/jci103746
Abstract
A method of graded osmotic hemolysis is described which achieves a separation of the whole erythrocyte population into fractions of differing mean cell age. This method was validated by employing Fe59 to label, in vivo, erythrocytes of a limited age. The young human erythrocytes were found to be more resistant to hemolysis in hypotonic media than older cells. It is suggested that this method may be useful in studies of biochemical changes associated with in vivo aging of erythrocytes.Keywords
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