Optimal Population of FoxP3+ T Cells in Tumors Requires an Antigen Priming-Dependent Trafficking Receptor Switch
Open Access
- 23 January 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (1), e30793
- https://doi.org/10.1371/journal.pone.0030793
Abstract
FoxP3+ T cells populate tumors and regulate anti-tumor immunity. The requirement for optimal population of FoxP3+ regulatory T cells in tumors remains unclear. We investigated the migration requirement and stability of tumor-associated FoxP3+ T cells. We found that only memory, but not naïve, FoxP3+ T cells are highly enriched in tumors. Almost all of the tumor-infiltrating FoxP3+ T cells express Helios, an antigen associated either with thymus-generated FoxP3+ T cells or activated T cells in the periphery. The tumor-infiltrating FoxP3+ T cells largely lack CD62L and CCR7, two trafficking receptors required for T cell migration into secondary lymphoid tissues. Instead, the tumor infiltrating FoxP3+ T cells highly express memory/tumor-associated CCR8 and CXCR4. Antigen priming is required for induction of this trafficking receptor phenotype in FoxP3+ T cells and only antigen primed, but not antigen-inexperienced naive, FoxP3+ T cells can efficiently migrate into tumors. While the migration of FoxP3+ T cells into tumors was a readily detectable event, generation of induced FoxP3+ T cells within tumors was unexpectedly inefficient. Genetic marking of current and ex-FoxP3+ T cells revealed that tumor-infiltrating FoxP3+ T cells are highly stable and do not readily convert back to FoxP3− T cells. Taken together, our results indicate that population of tumors with thymus-generated FoxP3+ T cells requires an antigen priming-dependent trafficking receptor switch in lymphoid tissues.Keywords
This publication has 58 references indexed in Scilit:
- Control of TH17/Treg Balance by Hypoxia-Inducible Factor 1Cell, 2011
- HIF1α–dependent glycolytic pathway orchestrates a metabolic checkpoint for the differentiation of TH17 and Treg cellsThe Journal of Experimental Medicine, 2011
- Instability of the transcription factor Foxp3 leads to the generation of pathogenic memory T cells in vivoNature Immunology, 2009
- The roles of CCR6 in migration of Th17 cells and regulation of effector T-cell balance in the gutMucosal Immunology, 2009
- CD4+FoxP3+ regulatory T cells confer infectious tolerance in a TGF-β–dependent mannerThe Journal of Experimental Medicine, 2008
- The Foxp3+ regulatory T cell: a jack of all trades, master of regulationNature Immunology, 2008
- Localisation pattern of Foxp3+ regulatory T cells is associated with clinical behaviour in gastric cancerBritish Journal of Cancer, 2007
- Melanoma Induces Immunosuppression by Up-Regulating FOXP3+ Regulatory T CellsJournal of Surgical Research, 2007
- The impact of regulatory T cells on carcinogen-induced sarcogenesisBritish Journal of Cancer, 2007
- Foxp3 in control of the regulatory T cell lineageNature Immunology, 2007