Effect of aluminium on iron uptake and transferrin-receptor expression by human erythroleukaemia K562 cells
- 1 December 1990
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 272 (2), 377-382
- https://doi.org/10.1042/bj2720377
Abstract
Incubation of human erythroleukaemia K562 cells with Al-transferrin inhibited iron uptake from 59Fe-transferrin by about 80%. The inhibition was greater than that produced by a similar quantity of Fe-transferrin. Preincubation of cells for 6 h with either Al-transferrin or Fe-transferrin diminished the number of surface transferrin receptors by about 40% compared with cells preincubated with apo-transferrin. Al-transferrin did not compete significantly with Fe-transferrin for transferrin receptors and, when cells were preincubated for 15 min instead of 6 h, the inhibitory effect of Al-transferrin on receptor expression was lost. Both forms of transferrin also decreased the level of transferrin receptor mRNA by about 50%, suggesting a common regulatory mechanism. Aluminium citrate had no effect on iron uptake or transferrin-receptor expression. AlCl3 also had no effect on transferrin-receptor expression, but at high concentration it caused an increase in iron uptake by an unknown, possibly non-specific, mechanism. Neither Al-transferrin nor AlCl3 caused a significant change in cell proliferation. It is proposed that aluminium, when bound to transferrin, inhibits iron uptake partly by down-regulating transferrin-receptor expression and partly by interfering with intracellular release of iron from transferrin.This publication has 23 references indexed in Scilit:
- Aluminum inhibits hemoglobin synthesis but enhances iron uptake in friend erythroleukemia cellsKidney International, 1990
- Aluminum inhibits erythropoiesis in vitro.JCI Insight, 1988
- Physiological and non-physiological forms of iron affect differently proliferation and ferritin synthesis in human mononuclear cells in vitro.The Tohoku Journal of Experimental Medicine, 1987
- Effects of different transferrin forms on transferrin receptor expression, iron uptake, and cellular proliferation of human leukemic HL60 cells. Mechanisms responsible for the specific cytotoxicity of transferrin-gallium.JCI Insight, 1986
- Metabolism and possible health effects of aluminum.Environmental Health Perspectives, 1986
- Aluminum ions stimulate mitosis in murine cells in tissue cultureJournal of Cellular Biochemistry, 1986
- Plasma aluminum is bound to transferrinLife Sciences, 1983
- Dialysis encephalopathy, bone disease and anaemia: the aluminum intoxication syndrome during regular haemodialysis.Journal of Clinical Pathology, 1981
- Alzheimer's Disease: X-Ray Spectrometric Evidence of Aluminum Accumulation in Neurofibrillary Tangle-Bearing NeuronsScience, 1980
- Iron uptake by Chang cells from transferrin, nitriloacetate and citrate complexesThe effects of iron-loading and chelation with desferrioxamineBiochimica et Biophysica Acta (BBA) - General Subjects, 1978