Reduction ofListeria monocytogeneson the Surface of Fresh Channel Catfish Fillets by Bacteriophage Listex P100
- 1 April 2010
- journal article
- research article
- Published by Mary Ann Liebert Inc in Foodborne Pathogens & Disease
- Vol. 7 (4), 427-434
- https://doi.org/10.1089/fpd.2009.0432
Abstract
Bacteriophage Listex P100 (phage P100) was approved by the U.S. Food and Drug Administration and U.S. Department of Agriculture's Food Safety and Inspection Service for Listeria monocytogenes control on both raw and ready-to-eat food products. In this article, we present the proof of concept on the influence of phage dose, phage contact time, and storage temperature on the listericidal activity of phage P100 in reducing the L. monocytogenes loads on the surface of fresh channel catfish fillet. The fresh catfish fillet samples were surface inoculated with ∼4.3 log10 colony forming units (CFU)/g of a two serotype mix (1/2a and 4b) of L. monocytogenes cells and then surface treated with phage P100. L. monocytogenes reduction was influenced by phage contact time and phage dose regardless of higher or lower temperature regimes tested on catfish fillet. The reduction in L. monocytogenes loads (p < 0.05) with the phage P100 dose of 2 × 107 plaque forming units (PFU)/g (7.3 log10 PFU/g) was 1.4–2.0 log10 CFU/g at 4°C, 1.7–2.1 log10 CFU/g at 10°C, and 1.6–2.3 log10 CFU/g at room temperature (22°C) on raw catfish fillet. The phage contact time of 30 min was adequate to yield greater than 1 log10 CFU/g reduction in L. monocytogenes, whereas 15 min contact time with phage yielded less than 1 log10 CFU/g reduction in L. monocytogenes loads on catfish fillet. Phage P100 titer was stable on catfish fillet samples, and overall reductions in L. monocytogenes counts were still maintained over a 10-day shelf life at 4°C or 10°C by phage P100 treatment. These findings illustrate the effectiveness of an alternative generally recognized as safe antimicrobial such as bacteriophage Listex P100 in quantitatively reducing L. monocytogenes from fresh catfish fillet surfaces.Keywords
This publication has 34 references indexed in Scilit:
- Role of Cold Shock Proteins in Growth of Listeria monocytogenes under Cold and Osmotic Stress ConditionsApplied and Environmental Microbiology, 2009
- Virulent Bacteriophage for Efficient Biocontrol of Listeria monocytogenes in Ready-To-Eat FoodsApplied and Environmental Microbiology, 2009
- Phage inactivation of foodborne pathogens on cooked and raw meatFood Microbiology, 2007
- Evaluation of a Cocktail of Three Bacteriophages for Biocontrol of Escherichia coli O157:H7Applied and Environmental Microbiology, 2004
- Characterization of virulent and avirulent Listeria monocytogenes strains by PCR amplification of putative transcriptional regulator and internalin genesJournal of Medical Microbiology, 2003
- IMPEDANCE MICROBIOLOGY TO SCREEN VARIOUS ANTIMICROBIALS ON WHOLE AND FILLET CHANNEL CATFISHJournal of Rapid Methods and Automation in Microbiology, 2003
- Ability of theListeria monocytogenesStrain Scott A To Cause Systemic Infection in Mice Infected by the Intragastric RouteApplied and Environmental Microbiology, 2002
- Effect of lactic acid on Listeria monocytogenes and Edwardsiella tarda attached to catfish skinFood Microbiology, 2001
- Combined antimicrobial effect of nisin and a listeriophage against Listeria monocytogenes in broth but not in buffer or on raw beefInternational Journal of Food Microbiology, 2001
- A Rapid Surface Intervention Process to Kill Listeria innocua on Catfish Using Cycles of Vacuum and SteamJournal of Food Science, 2001