Effects of adenovirus infection on rRNA synthesis and maturation in HeLa cells.
Open Access
- 1 April 1983
- journal article
- Published by Informa UK Limited in Molecular and Cellular Biology
- Vol. 3 (4), 662-671
- https://doi.org/10.1128/mcb.3.4.662
Abstract
The production of cytoplasmic and nucleolar rRNA species was examined in HeLa cells infected with high multiplicities of adenovirus type 5. Both 28S and 18S rRNA newly synthesized in infected cells ceased to enter the cytoplasm as reported previously (N. Ledinko, Virology 49: 79-89, 1972; H. J. Raskas, D. C. Thomas, and M. Green, Virology 40: 893-902, 1970). However, the effects on 28S cytoplasmic rRNA were observed considerably earlier in the infectious cycle than those on 18S rRNA. The inhibition of cellular protein synthesis and of the appearance in the cytoplasm of labeled cellular mRNA sequences (G. A. Beltz and S. J. Flint, J. Mol. Biol. 131: 353-373, 1979) were also monitored in infected cultures. During the later periods of an infectious cycle, from 18 h after infection, nucleolar rRNA synthesis and processing and exit of 18S rRNA from the nucleus were inhibited, probably reflecting the failure of infected cells to synthesize normal quantities of ribosomal proteins. The earliest responses of cellular RNA metabolism to adenovirus infection were, however, the rapid and apparently coordinate reductions in the levels of newly synthesized 28S rRNA and cellular mRNA sequences entering the cytoplasm.Keywords
This publication has 35 references indexed in Scilit:
- Processing of 45 s nucleolar RNAJournal of Molecular Biology, 1970
- Effects of valine deprivation on ribosome formation in HeLa cellsJournal of Molecular Biology, 1969
- Biochemical studies on adenovirus multiplicationVirology, 1969
- The polypeptides of adenovirusVirology, 1969
- THE REGULATION OF RNA SYNTHESIS AND PROCESSING IN THE NUCLEOLUS DURING INHIBITION OF PROTEIN SYNTHESISThe Journal of cell biology, 1969
- Synthesis of ribosomal proteins in the absence of ribosome maturation in methionine-deficient HeLa cellsJournal of Molecular Biology, 1968
- Selective suppression of nucleolar RNA metabolism in the absence of protein synthesisBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- The effects of methionine deprivation on ribosome synthesis in HeLa cells.Proceedings of the National Academy of Sciences of the United States of America, 1967
- Acrylamide gel electrophoresis of HeLa cell nucleolar RNA.Proceedings of the National Academy of Sciences of the United States of America, 1967
- Ribosome formation in HeLa cells in the absence of protein synthesisJournal of Molecular Biology, 1966