Association of hepatitis C virus carrier state with the occurrence of hepatitis C virus core antibodies
- 1 April 1992
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 36 (4), 259-264
- https://doi.org/10.1002/jmv.1890360405
Abstract
An enzyme immunoassay (EIA) was developed for the determination of antibodies against the “putative” core protein of hepatitis C virus (HCV). Antigens used were recombinant fragments (amino acids 6-77 or 6-143) of the HCV core protein, produced in Escherichia coli with truncated hepatitis B core (HBc) as fusion protein. Evaluation of 385 sera positive for HCV antibodies by first generation EIA, revealed 98 (25.4%) with HCV core antibodies. HCV-RNA, determined by the polymerase chain reaction (PCR), was exclusively found in the sera positive for HCV core antibodies (89 PCR positives). In random screening of 3,708 sera, 3 sera with HCV core antibodies were found PCR positive. Only 2 of these sera were positive in the first generation EIA It is concluded that HCV core antibody determination is a reliable test for identifying HCV carriers among blood donors.Keywords
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