Rational Design of Nanocarriers for Intracellular Protein Delivery
Top Cited Papers
- 8 September 2019
- journal article
- review article
- Published by Wiley in Advanced Materials
- Vol. 31 (46), e1902791
- https://doi.org/10.1002/adma.201902791
Abstract
Protein/antibody therapeutics have exhibited the advantages of high specificity and activity even at an extremely low concentration compared to small molecule drugs. However, they are accompanied by unfavorable physicochemical properties such as fragile tertiary structure, large molecular size, and poor penetration of the membrane, and thus the clinical use of protein drugs is hindered by inefficient delivery of proteins into the host cells. To overcome the challenges associated with protein therapeutics and enhance their biopharmaceutical applications, various protein‐loaded nanocarriers with desired functions, such as lipid nanocapsules, polymeric nanoparticles, inorganic nanoparticles, and peptides, are developed. In this review, the different strategies for intracellular delivery of proteins are comprehensively summarized. Their designed routes, mechanisms of action, and potential therapeutics in live cells or in vivo are discussed in detail. Furthermore, the perspective on the new generation of delivery systems toward the emerging area of protein‐based therapeutics is presented as well.Keywords
Funding Information
- National Natural Science Foundation of China (81672508, 61601218)
- Natural Science Foundation of Shaanxi Province (2019JM‐016)
- National Research Foundation Singapore (SBP‐P4, SBP‐P8)
- Fundamental Research Funds for the Central Universities
- Natural Science Foundation of Guangdong Province (2017A030313299)
This publication has 266 references indexed in Scilit:
- Genetically Engineered Microvesicles Carrying Suicide mRNA/Protein Inhibit Schwannoma Tumor GrowthMolecular Therapy, 2013
- Improving the Endosomal Escape of Cell-Penetrating Peptides and Their Cargos: Strategies and ChallengesPharmaceuticals, 2012
- The targeted intracellular delivery of cytochrome C protein to tumors using lipid-apolipoprotein nanoparticlesBiomaterials, 2012
- Monolayer coated gold nanoparticles for delivery applicationsAdvanced Drug Delivery Reviews, 2011
- Self-assembling elastin-like peptides growth factor chimeric nanoparticles for the treatment of chronic woundsProceedings of the National Academy of Sciences, 2010
- Potent Delivery of Functional Proteins into Mammalian Cells in Vitro and in Vivo Using a Supercharged ProteinACS Chemical Biology, 2010
- Intracellular Delivery of a Membrane-Impermeable Enzyme in Active Form Using Functionalized Gold NanoparticlesJournal of the American Chemical Society, 2010
- Mechanism of active targeting in solid tumors with transferrin-containing gold nanoparticlesProceedings of the National Academy of Sciences, 2009
- Nanoparticle interaction with plasma proteins as it relates to particle biodistribution, biocompatibility and therapeutic efficacyAdvanced Drug Delivery Reviews, 2009
- Renal clearance of quantum dotsNature Biotechnology, 2007