Diagnosing breast cancer using Raman spectroscopy: prospective analysis
- 1 January 2009
- journal article
- research article
- Published by SPIE-Intl Soc Optical Eng in Journal of Biomedical Optics
- Vol. 14 (5), 054023-054023-8
- https://doi.org/10.1117/1.3247154
Abstract
We present the first prospective test of Raman spectroscopy in diagnosing normal, benign, and malignant human breast tissues. Prospective testing of spectral diagnostic algorithms allows clinicians to accurately assess the diagnostic information contained in, and any bias of, the spectroscopic measurement. In previous work, we developed an accurate, internally validated algorithm for breast cancer diagnosis based on analysis of Raman spectra acquired from fresh-frozen in vitro tissue samples. We currently evaluate the performance of this algorithm prospectively on a large ex vivo clinical data set that closely mimics the in vivo environment. Spectroscopic data were collected from freshly excised surgical specimens, and 129 tissue sites from 21 patients were examined. Prospective application of the algorithm to the clinical data set resulted in a sensitivity of 83%, a specificity of 93%, a positive predictive value of 36%, and a negative predictive value of 99% for distinguishing cancerous from normal and benign tissues. The performance of the algorithm in different patient populations is discussed. Sources of bias in the in vitro calibration and ex vivo prospective data sets, including disease prevalence and disease spectrum, are examined and analytical methods for comparison provided.Keywords
This publication has 33 references indexed in Scilit:
- Prospects for the diagnosis of breast cancer by noninvasive probing of calcifications using transmission Raman spectroscopyJournal of Biomedical Optics, 2007
- Determination of uncertainty in parameters extracted from single spectroscopic measurementsJournal of Biomedical Optics, 2007
- Differences in outcome for positive margins in a large cohort of breast cancer patients treated with breast-conserving therapyActa Oncologica, 2007
- Diagnosis of breast cancer using diffuse reflectance spectroscopy: Comparison of a Monte Carlo versus partial least squares analysis based feature extraction techniqueLasers in Surgery and Medicine, 2006
- Quantitative spectroscopic diffuse optical tomography of the breast guided by imperfecta prioristructural informationPhysics in Medicine & Biology, 2005
- Practical Considerations in the Pathologic Diagnosis of Needle Core Biopsies of BreastAmerican Journal of Clinical Pathology, 2002
- Model-based biological Raman spectral imagingJournal of Cellular Biochemistry, 2002
- Raman Spectroscopy and Fluorescence Photon Migration for Breast Cancer Diagnosis and ImagingPhotochemistry and Photobiology, 1998
- Identification and Quantification of Intrinsic Optical Contrast for Near‐infrared MammographyPhotochemistry and Photobiology, 1998
- pathological prognostic factors in breast cancer. I. The value of histological grade in breast cancer: experience from a large study with long‐term follow‐upHistopathology, 1991