Quantification of Deficits in Spatial Visual Function of Mouse Models for Glaucoma
Open Access
- 1 May 2011
- journal article
- glaucoma
- Published by Association for Research in Vision and Ophthalmology (ARVO) in Investigative Opthalmology & Visual Science
- Vol. 52 (6), 3654-3659
- https://doi.org/10.1167/iovs.10-7106
Abstract
Purpose.: DBA/2J mice are a standard preclinical glaucoma model, which spontaneously developed mutations resulting in chronic age-related pigmentary glaucoma. The goals of this study were to identify the degree of visual impairment in DBA/2J mice before and after disease onset by quantifying the optokinetic reflex responses and to compare them to the less-researched strain of DBA/2NHsd mice. Methods.: Visual performance was measured in healthy, nonglaucomatous, and glaucomatous male DBA/2NHsd or DBA/2J mice using a visuospatial testing box. The optokinetic reflex resulting in optomotor head tracking was manually detected. Measured threshold levels equate to the maximum contrast or spatial frequency the mouse responds to. Intraocular pressure (IOP) was measured by applanation tonometry. Results.: IOP increased with age in both DBA/2J and DBA/2NHsd mice and was not different between the two substrains. Both visual acuity and ability to detect contrast decreased significantly, and similarly with age in both substrains. However, DBA/2NHsd had poorer visual acuity even at a younger age compared to age-matched DBA/2J mice. Conclusions.: Both DBA/2J and DBA/2NHsd mice show a progressive glaucomatous phenotype of age-related increases in IOP and loss of visual acuity and contrast sensitivity when compared to other inbred or outbred strains. Given the similar increases in IOP and contrast sensitivity threshold and loss of visual acuity between these two DBA/2 substrains, we also conclude that DBA/2NHsd mice are a suitable alternative model for pigmentary glaucoma.Keywords
This publication has 39 references indexed in Scilit:
- Neurodegeneration in glaucoma: progression and calcium-dependent intracellular mechanismsNeuroscience, 2010
- Murine models of acute and chronic lung infection with cystic fibrosis pathogensInternational Journal of Medical Microbiology, 2010
- Rapid Quantification of Adult and Developing Mouse Spatial Vision Using a Virtual Optomotor SystemPublished by Association for Research in Vision and Ophthalmology (ARVO) ,2004
- Tonopen measurement of intraocular pressure in miceExperimental Eye Research, 2004
- Retinal neurodegeneration in the DBA/2J mouse?a model for ocular hypertensionActa Neuropathologica, 2004
- Retinal morphology and ERG response in the DBA/2NNia mouse model of angle-closure glaucoma.2001
- Comparison of contrast sensitivity, visual acuity, and Humphrey visual field testing in patients with glaucoma.2001
- Visual acuity versus letter contrast sensitivity in early cataractVision Research, 1998
- Essential iris atrophy, pigment dispersion, and glaucoma in DBA/2J mice.1998
- Correlation of spatial contrast sensitivity and visual fields in glaucomaAlbrecht von Graefes Archiv für Ophthalmologie, 1993